Publications

We publish our research in pre-prints and leading journals within the fields of synthetic biology for natural products, cell-free synthetic biology, and antimicrobial resistance. We are very grateful to our funding bodies for their continued support. Past and present funders include the Leverhulme Trust, BBSRC, Royal Society, Wellcome Trust, FAPESP (São Paulo), Biochemical Society, Syngenta, UKRI-sponsored networks (High-Value Biorenewables, Future Targeted Healthcare and Manufacturing Hub) and industry. We are committed to delivering impactful research that advances scientific knowledge and addresses real-world challenges.

28.

Sebastián Bermúdez-Puga, Bruno Mendes, Jean Pierre Ramos-Galarza, Pamela Oliveira de Souza de Azevedo, Attilio Converti, Francesco Molinari, Simon J Moore, José R Almeida, Ricardo Pinheiro de Souza Oliveira

Revolutionizing agroindustry: Towards the industrial application of antimicrobial peptides against pathogens and pests

Biotechnology Advances (2025) 82:108605

DOI

27.

Andrew J. Rice, Tien T. Sword, Kameshwari Chengan, Douglas A. Mitchell, Nigel J. Mouncey, Simon J. Moore and Constance B. Bailey

Cell-free synthetic biology for natural product biosynthesis and discovery

Chemical Society Reviews (2025) 54:4314-4352

DOI

26.

Hung-En Lai, Agata Kennedy, Lewis Tanner, Emma A. Bartram, Soo Mei Chee, Paul S. Freemont, Simon J. Moore

Biosynthesis of Arcyriaflavin F from Streptomyces venezuelae ATCC 10712

ChemBioChem (2024) 25:e202400357

DOI

25.

Sebastián Bermúdez-Puga, Meriellen Dias, Iara Lima Reis, Taciana Freire de Oliveira, Sonia Regina Yokomizo de Almeida, Maria Anita Mendes, Simon J Moore, José R Almeida, Carolina Proano-Bolanos, Ricardo Pinheiro de Souza Oliveira

Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins

Biochimie (2024) 225:168-175

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24.

Kameshwari Chengan, Charlotte Hind, Maria Stanley, Matthew E Wand, Lakshmeesha K Nagappa, Kevin Howland, Tanith Hanson, Rubén Martín-Escolano, Anastasios D Tsaousis, José A Bengoechea, J Mark Sutton, Christopher M Smales, Simon J Moore

A cell-free strategy for the host-specific profiling of antimicrobial sensitivity and resistance

npj Antimicrobials and Resistance 1:16 (2023)

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23.

Simon J. Moore, Hung-En Lai, Jian Li and Paul S. Freemont

Streptomyces cell-free systems for natural product discovery and engineering

Nat. Prod. Rep. (2023) 40:228-236

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22.

Lakshmeesha K. Nagappa, Wakana Sato, Farzana Alam, Kameshwari Chengan, Christopher M. Smales, Tobias Von Der Haar, Karen M. Polizzi, Katarzyna P. Adamala and Simon J. Moore

A ubiquitous amino acid source for prokaryotic and eukaryotic cell-free transcription-translation systems

Front. Bioeng. Biotechnol. (2022)10:992708

DOI

21.

Agata Kennedy, Guy Griffin, Paul S. Freemont, Karen M. Polizzi, and Simon J. Moore

A curcumin direct protein (DiPro) biosensor for cell-free prototyping

Engineering Biology 6(2):62-68 (2022)

DOI

20.

Jian Li, Yong-Chan Kwon, Yuan Lu, Simon J. Moore

Editorial: Cell-Free Synthetic Biology

Frontiers in Bioengineering and Biotechnology 9:1210 (2022)

DOI

19.

Hung-En Lai, Alan M. C. Obled, Soo Mei Chee, Rhodri M. Morgan, Rosemary Lynch, Sunil V. Sharma, Simon J. Moore, Karen M. Polizzi, Rebecca J. M. Goss, and Paul S. Freemont

A GenoChemetic strategy for derivatization of the violacein natural product scaffold

ACS Chemical Biology (2021) 16(11):2116

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18.

Ming Toh, Kameshwari Chengan, Tanith Hanson, Paul S. Freemont, and Simon J. Moore

A high-yield Streptomyces transcription-translation toolkit for synthetic biology and natural product applications

JoVE (2021) 10.3791/63012

DOI

17.

Simon J. Moore, Tomasso Tosi, David J. Bell, Yonek Hleba, Karen M. Polizzi, and Paul S Freemont

High-yield 'one-pot' biosynthesis of raspberry ketone, a high-value fine chemical

Synth. Biol. (2021) 6:ysab021

DOI

16.

Simon J. Moore, Yonek B Hleba, Sarah Bischoff, David J. Bell, Karen M. Polizzi, Paul S. Freemont

Refactoring of a Synthetic Raspberry Ketone Pathway with EcoFlex

Microb. Cell Factories (2021) 20: 116

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15.

Simon J. Moore, Hung-En Lai, Soo-Mei Chee, Ming Toh, Seth Coode, Kameshwari Chengan, Patrick Capel, Christophe Corre, Emmanuel de Los Santos, and Paul S. Freemont

A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology

ACS Synth. Biol. (2021) 10: 402-411

DOI

14.

Simon J. Moore

Enzyme alchemy: cell-free synthetic biochemistry for natural products

Emerging Topics in Biosciences (2019) 3: 529-535

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13.

Hung-En Lai, Caoimhe Canavan, Loren Cameron, Simon J. Moore, Monika Danchenko, Todd Kuiken, Zuzana Sekeyova, and Paul S. Freemont

Synthetic biology and the United Nations

Trends in Biotechnology (2019) 37: 1146-1151

DOI

12.

Simon J. Moore, James T. MacDonald, Sarah Wienecke, Alka Ishwarbhai, Argyro Tsipa, Rochelle Aw, Nicolas Kylilis, David J. Bell, David W. McClymont, Kirsten Jensen, Karen M. Polizzi, Rebekka Biedendieck, and Paul S. Freemont

Rapid acquisition and model-based analysis of cell-free transcription-translation reactions from non-model bacteria

Proceedings of the National Academy of Sciences, USA (2018) 115:E4340-E4349

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11.

Simon J. Moore, James T. MacDonald, and Paul S. Freemont

Cell-free synthetic biology for in vitro prototype engineering

Biochemical Society Transactions (2017) 45:785-791

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10.

Simon J. Moore, Sven T. Sowa, Christopher Schuchardt, Evelyne Deery, Andrew D. Lawrence, Jose Vazquez Ramos, Susan Billig, Claudia Birkemeyer, Peter T. Chivers, Mark J. Howard, Stephen E. J. Rigby, Gunhild Layer, and Martin J. Warren

Elucidation of the biosynthesis of the methane catalyst coenzyme F430

Nature (2017) 543:78-82

DOI

Simon J. Moore, Hung-En Lai, Hannah Needham, Karen M. Polizzi, and Paul S. Freemont

Streptomyces venezuelae TX-TL - a next generation cell-free synthetic biology tool

Biotechnol J (2017) DOI: 10.1002/biot.201600678

DOI

Simon J. Moore, Hung-En Lai, Richard J. R. Kelwick, Soo Mei Chee, David J Bell, Karen M. Polizzi, and Paul S. Freemont

EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology

ACS Synth. Biol. (2016) 5:1059-1069

DOI

Margarita B. Kopniczky, Simon J. Moore, and Paul S. Freemont

Multilevel regulation and translational switches in synthetic biology

IEEE Transactions (2015) 9:485-496

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Simon J. Moore, Matthias J. Mayer, Rebekka Biedendieck, Evelyne Deery, and Martin J. Warren

Towards a cell factory for vitamin B12 production in Bacillus megaterium: Bypassing of the cobalamin riboswitch control elements

New Biotechnology (2014) 31:553-61

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Simon J. Moore, Andrew D. Lawrence, Rebekka Biedendieck, Evelyne Deery, Stefanie Frank, Mark J. Howard, Stephen E. J. Rigby, and Martin J. Warren

Elucidation of the anaerobic pathway for the corrin component of cobalamin (vitamin B12)

PNAS (2013) 110:14906-14911

DOI

Simon J. Moore, Rebekka Biedendieck, Andrew D. Lawrence, Evelyne Deery, Mark J. Howard, Stephen E. J. Rigby, and Martin J. Warren

Characterization of the enzyme CbiH60 involved in anaerobic ring contraction of the cobalamin (vitamin B12) biosynthetic pathway

Journal of Biological Chemistry (2013) 288:297-305

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Simon J. Moore and Martin J. Warren

The anaerobic biosynthesis of vitamin B12

Biochemical Society Transactions (2012) 40: 581-586

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Book Chapters

Evelyne Deery, Stefanie Frank, Andrew D. Lawrence, Simon J. Moore, Susanne Schroeder, and Martin J. Warren

Synthetic biology and a new era in metabolic engineering: From complex biochemical pathways to compartmentalised metabolic processes - a vitamin connection

Encyclopedia of Molecular Cell Biology and Molecular Medicine, Wiley (2014)

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Hung-En Lai, Simon J. Moore, Karen M. Polizzi, and Paul S. Freemont

EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology

Springer Protocols: Synthetic Biology (2018) 1772:429-444

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